Sugar solution makes tissues see-through

Japanese researchers have developed a new sugar and water-based solution that turns tissues transparent in just three days, without disrupting the shape and chemical nature of the samples. Combined with fluorescence microscopy, this technique enabled them to obtain detailed images of a mouse brain at an unprecedented resolution.

The team from the RIKEN Center for Developmental biology reports their finding today in Nature Neuroscience.

Over the past few years, teams in the USA and Japan have reported a number of techniques to make biological samples transparent, that have enabled researchers to look deep down into biological structures like the brain.

“However, these clearing techniques have limitations because they induce chemical and morphological damage to the sample and require time-consuming procedures,” explains Dr. Takeshi Imai, who led the study.

SeeDB, an aqueous fructose solution that Dr. Imai developed with colleagues Drs. Meng-Tsen Ke and Satoshi Fujimoto, overcomes these limitations.

Using SeeDB, the researchers were able to make mouse embryos and brains transparent in just three days, without damaging the fine structures of the samples, or the fluorescent dyes they had injected in them.

They could then visualize the neuronal circuitry inside a mouse brain, at the whole-brain scale, under a customized fluorescence microscope without making mechanical sections through the brain.

They describe the detailed wiring patterns of commissural fibers connecting the right and left hemispheres of the cerebral cortex, in three dimensions, for the first time. They also report that they were able to visualize in three dimensions the wiring of mitral cells in the olfactory bulb, which is involved the detection of smells, at single-fiber resolution.

“Because SeeDB is inexpensive, quick, easy and safe to use, and requires no special equipment, it will prove useful for a broad range of studies, including the study of neuronal circuits in human samples,” explain the authors.

Image 1: Mouse embryo and mouse brain before and after treatment with SeeDB. Image 2: Seeing through a 6 mm-thick mouse brain thanks to SeeDB combined with two-photon microscopy

(Source: riken.jp)

  1. wrathfuldaughter reblogged this from neuromorphogenesis
  2. iwillgetbacktoyouok reblogged this from scinerds
  3. dead--and--alive reblogged this from neuromorphogenesis
  4. cyrrane reblogged this from zakahrumite
  5. scholasticendeavors reblogged this from scorpshirl
  6. scorpshirl reblogged this from scinerds
  7. microkrill reblogged this from neuromorphogenesis
  8. regalgypsy reblogged this from lvtrndtkm
  9. gloriousfindel reblogged this from damnitwhatisthecatdoing
  10. talesofscienceandlove reblogged this from molecularlifesciences
  11. i-dont-need-anyone-now reblogged this from scinerds
  12. thebestsometimesforget reblogged this from molecularlifesciences
  13. tenderandfire reblogged this from scinerds
  14. aid-an reblogged this from neuromorphogenesis
  15. sk8er reblogged this from neuromorphogenesis
  16. wojojojo reblogged this from neuromorphogenesis
  17. nekkidintheflood reblogged this from neuromorphogenesis
  18. individualityambitionmotivation reblogged this from molecularlifesciences and added:
    Neuromorphogenesis…Yeah, science ;)
  19. egyptiianmau reblogged this from neuromorphogenesis
  20. danslefeudelaction reblogged this from cdnabroad
  21. teajing reblogged this from scinerds
  22. rosesrage reblogged this from scinerds
  23. tabernil reblogged this from molecularlifesciences
  24. theconsultingchemist reblogged this from neuromorphogenesis
  25. robote reblogged this from scinerds
  26. naughtywheniwannabe reblogged this from scinerds
  27. gowitheflow reblogged this from neuromorphogenesis